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| Registros recuperados: 89 | |
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| Pi,Yan; Jiang,Keji; Hou,Rong; Gong,Yifu; Lin,Juan; Sun,Xiaofen; Tang,Kexuan. |
| Camptothecin and its derivatives are monoterpenoid indole alkaloids exhibiting significant anti-tumor actions. With the aim of improving the production of these pharmaceuticals, the contents of camptothecin and 10-hydroxycamptothecin in different tissues including roots, stems, leaves, young flower buds, opening flowers, fading flowers and seeds from Camptotheca acuminata, were investigated. The young flower buds had the highest alkaloid concentrations (camptothecin, 2.46 mg/g of dry weight; 10-hydroxycamptothecin, 1.41 mg/g of dry weight). Callus showed lower concentrations but it should also be considered as a potential source of these pharmaceuticals. In the present study, the growth rate of Camptotheca acuminata cells in culture did not correlate with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HPLC; Salicylic acid; UV-B. |
| Ano: 2010 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000300006 |
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| RESENDE,FERNANDA B.S.; LIRA,LARISSA Q. DE; GRILO,EVELLYN C.; LIMA,MAYARA S.R.; DIMENSTEIN,ROBERTO. |
| The influence of gestational diabetes on vitamin A deficiency in lactating women and, consequently, in their newborn has been verified through a cross-sectional case-control study conducted with volunteer puerperal women. The control group consisted of healthy women and the test group was composed of women with gestational diabetes. One hundred and seven women were recruited, corresponding to 71 controls and 36 cases. Personal, gestational and newborn data were collected directly from medical records during hospitalization. The retinol was determined in maternal colostrum and serum by High Performance Liquid Chromatography. Postpartum women with gestational diabetes were older, had more children and a higher prevalence of cesarean delivery. No difference... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Colostrum; Gestational diabetes; HPLC; Hypovitaminosis A; Postpartum. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100463 |
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| Matallo,M.B.; Almeida,S.D.B.; Franco,D.A.S.; Cerdeira,A.L.; Gazzeiro,D.L.P.. |
| Oseltamivir phosphate is a potent viral inhibitor produced from shikimic acid extracted from seeds of Ilicium verum, the most important natural source. With the site of action 5-enolpyruvylshikimate-3-phosphate synthase (EPSP), glyphosate is the only compound capable of inhibiting its activity with the consequent accumulation of shikimic acid in plants. Corn and soybean plants were sprayed with reduced rates of glyphosate (0.0 to 230.4 g a.i. ha¹) and shikimic acid content in the dry mass was determined by HPLC 3, 7 and 10 days after application. Results showed shikimic acid accumulation in dry mass with increases of up to 969% in corn and 33,000% on soybeans, with peak concentrations 3 days after treatment (DAT). Industrial feasibility for shikimic acid... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Herbicides; Corn; Soybean; Oseltamivir; Pandemics; HPLC. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582014000300016 |
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| Leite,R.M.H.; Leite,R.C.; Lima,J.A.; Fóscolo,C.B.; Mota,P.M.P.C.; Lobato,F.C.F.; Lage,A.P.. |
| The high pressure liquid chromatography (HPLC) was used for the identification of isoniazid (isonicotinic acid hydrazide) in the milk of cattle treated with a dose of 25 mg/kg/day in alternated days. The effect of milk pasteurization on the isoniazid residue concentration was also studied. The drug excretion presented a cyclic variation, with higher levels in the first day after administration (aa), a mean of 1104.48µg/l, and a decrease two days aa, with a mean of 104.12µg/l. Four days after the last administration of the drug it was not possible to identify residues of isoniazid in the milk of treated animals. Body weight and milk yield influenced the amount of the excreted drug, and pasteurization decreased (mean 47.07%) the concentration of isoniazid... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cattle; Tuberculosis; Isoniazid; Milk; HPLC. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000600018 |
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| RODRIGUES, E. P.; COELHO, M. S.; SOARES, C. de P.; VIDAL, M. S.; ARAUJO, J. L. S. de; BALDANI, J. I.. |
| A espécie Gluconacetobacter diazotrophicus é uma bactéria endofítica reconhecida pela sua capacidade de fixação biológica de nitrogênio (FBN) em associação com a cultura de cana-de-açúcar. Além da FBN, a produção de auxinas tem sido considerada como um fator adicional na promoção do crescimento desta cultura. Neste estudo, foi descrita uma metodologia para a identificação e quantificação de compostos indólicos presentes no sobrenadante de Gluconacetobacter diazotrophicus, crescida em meio líquido, utilizando a técnica de cromatografia líquida de alta eficiência (HPLC) e extração em fase sólida (SPE). Com esta metodologia foi possível realizar a detecção por HPLC de ácido indol pirúvico no sobrenadante de G. diazotrophicus sugerindo que a rota do IPyA foi... |
| Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) |
Palavras-chave: Compostos indólicos; HPLC. |
| Ano: 2009 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/664190 |
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| Archegas,Lucí Regina Panka; Rached,Rodrigo Nunes; Ignacio,Sérgio Aparecido; Vasconcelos,Eliane Carvalho de; Ramos,Débora Toledo; Souza,Evelise Machado de. |
| The aim of this study was to detect and quantify the main residual monomers released from composites, using high performance liquid chromatography (HPLC). Discs were made with dental composites (Herculite XRV, Tetric Ceram and Filtek Z250) and immersed in deionized water at 37ºC for 28 days, with water changes in 1, 7, 14 and 21 days. The mean concentration of residual monomers were subject to the Kruskal-Wallis test (p<0.05). Tetric Ceram exhibited significantly higher concentrations of leached monomers. Bis-GMA was the monomer released in lower concentrations for all the materials. There was no statistical difference between the amounts of TEGDMA and UDMA. Most of the monomers demonstrated maximal concentration at the 7-day period. The HPLC analysis... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dental composites; Monomers; HPLC; TEGDMA; Bis-GMA; UDMA. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400009 |
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| Brotas, V; Plantecuny, Mr. |
| A HPLC protocol is described which permits analysis of the pigment composition of coastal and intertidal sediments. The HPLC technique has been extensively applied to phytoplankton pigments for fifteen years. Papers dealing with living benthic microphytic populations have been more rare, due to complexity existing in the sediment surface, where pigments (chlorophylls and carotenoids) from several taxonomic groups of microalgae are mixed with degraded pigments from senescent phytoplankton cells and macrophytic material. The present paper describes in detail a protocol adapted from Kraay et al. (1992), which allows a good discrimination of photosynthetic pigments of microphytobenthos communities. The following procedure is recommended. Sediment samples are... |
| Tipo: Text |
Palavras-chave: Plants pigments; Sediments; Protocol; HPLC. |
| Ano: 1996 |
URL: http://archimer.ifremer.fr/doc/00096/20730/18367.pdf |
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| Moukette,Bruno Moukette; Pieme,Constant Anatole; Njimou,Jacques Romain; Biapa,Cabral Prosper Nya; Marco,Bravi; Ngogang,Jeanne Yonkeu. |
| BACKGROUND: Excessive production of free radicals causes direct damage to biological molecules such as DNA, proteins, lipids, carbohydrates leading to tumor development and progression. Natural antioxidant molecules from phytochemicals of plant origin may directly inhibit either their production or limit their propagation or destroy them to protect the system. In the present study, Monodora myristica a non-timber forest product consumed in Cameroon as spice was screened for its free radical scavenging properties, antioxidant and enzymes protective activities. Its phenolic compound profile was also realized by HPLC. RESULTS: This study demonstrated that M. myristica has scavenging properties against DPPH',OH',NO', and ABTS'radicals which vary in a dose... |
| Tipo: Journal article |
Palavras-chave: Antioxidant; Radical scavenging; HPLC; Monodora myristica; Non-timber forest product. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100015 |
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| CASTRO,ANA HORTÊNCIA FONSÊCA; YOUNG,MARIA CLÁUDIA MARX; ALVARENGA,AMAURI ALVES DE; ALVES,JOSÉ DONIZETI. |
| In order to evaluate the influence of photoperiod on the accumulation of allantoin in comfrey (Symphytum officinale L.), a well known medicinal plant, an experiment was conducted during July and August, 1998. Cuttings obtained from 90 day old plants were submitted to four photoperiods (8, 12, 16 and 20 hours). After 60 days, allantoin content in roots and rhizomes was evaluated. The results showed that increases in photoperiod promoted an increment in the average content of allantoin in roots (0.06%, 0.303%, 1.213% and 4.78%). On the other hand, in rhizomes, allantoin accumulation decreased (9.65%, 7.14%, 0.55%) when the photoperiod was increased from 8 to 12 and 16 hours, respectively, stabilizing on a 20-hour photoperiod (0.53%). Plants cultivated under... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HPLC; Medicinal plant; Secondary metabolism; Symphytum officinale; Ureides. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-31312001000100006 |
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| EMSEN,BUGRAHAN; OZDEMIR,OZLEM; ENGIN,TUBANUR; TOGAR,BASAK; CAVUSOGLU,SEYDA; TURKEZ,HASAN. |
| Abstract: Herbal medicines are efficient to reduce side effects in the fight against glioblastoma, which plays a critical role within brain cancer species. The recent studies designated for testing the effects of lichens that have shown numerous anticancer activities on glioblastoma so far. In the present study, different concentrations of water extract obtained from Usnea longissima Ach. were used in order to determine cytotoxic (via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and lactate dehydrogenase tests), antioxidant (via total antioxidant capacity test), pro-oxidant (via total oxidant status test) and genotoxic (via 8-hydroxy-2′-deoxyguanosine test) effects of them on human U87MG-glioblastoma cancer cell lines. Primary mixed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cytotoxicity; Genotoxicity; HPLC; Total antioxidant capacity; Total oxidative stress. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500621 |
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| Cremonini,D.N.; Ranzani,J.J.T.; Cunha,M.L.R.S.; Tonin,F.G.; Brandão,C.V.S.; Messadeq,Y.. |
| Avaliou-se a concentração de ofloxacina liberada por uma lente de contato de membrana de celulose biossintética, para tratamento de ceratite bacteriana experimental em cães, pela inoculação de Staphylococcus aureus intraestromal. Comparou-se o tratamento com a lente de contato biossintética impregnada com ofloxacina à terapia tópica convencional. Realizou-se avaliação microbiológica e dosagem de ofloxacina no humor aquoso por meio do método de cromatografia líquida de alto rendimento (HPLC). Houve diferença estatística na contagem de colônias bacterianas entre os olhos com ceratite e os demais grupos, no primeiro dia de coleta. O biomaterial, impregnado com ofloxacina, promoveu liberação gradual durante o período de avaliação, aos três e sete dias; no... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cão; Ceratite bacteriana; Membrana biossintética; Ofloxacina; HPLC. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000400004 |
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| Souza,Adailson P. de; Prates,Hélio T.; Ferreira,Francisco A.; Reis,Efraim L.; Jordão,Claudio P.. |
| Em razão das poucas de informações existente na literatura, sobre a mobilidade do glyphosate e do imazapyr, em solos de baixa atividade, comum em países tropicais, foi conduzido, em laboratório, um estudo sobre a mobilidade vertical desses herbicida s em dois solos com diferente s composições químicas e fisicas. Para estudar essa mobilidade, foram utilizadas colunas de solo com diferentes alturas, as quais, após aplicação dos produtos na dose comerc ial de 4 L ha-1, foram submetida s a um regime hídrico de 40 mm h-1, por um período de 4 h. As análises dos resíduos nas diferentes profundidades foram feitas utilizando-se os métodos voltamétrico (polarografia) e HPLC, para o glyphosate e imazapyr, respectivame nte. Com base nos resultados, foi possível... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Herbicidas; Voltametria; Polarografia; Cromatografia; HPLC. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83581999000200009 |
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| Kraemer,Alejandro Fausto; Marchesan,Enio; Grohs,Mara; Avila,Luis Antonio de; Machado,Sérgio Luiz de Oliveira; Zanella,Renato; Massoni,Paulo Fabricio Sachet; Sartori,Gerson Meneghetti Sarzi. |
| O imazethapyr está sendo amplamente utilizado no Rio Grande do Sul desde o desenvolvimento da Tecnologia Clearfield® (CL) em arroz, em uma mistura formulada de imazethapyr + imazapic (75 + 25g L-1). No entanto, com o uso dessa tecnologia, surgiu o problema da persistência do herbicida no solo, afetando cultivos não tolerantes em sucessão com diferentes intensidades. A fitointoxicação está relacionada, dentre vários fatores, à localização do herbicida no perfil do solo. O presente trabalho teve por objetivo determinar o posicionamento do imazethapyr em profundidade, no perfil de um solo de várzea cultivado com arroz, frente a dois tipos de manejo. Foram coletadas amostras de solos, em diferentes profundidades (0-5, 5-10, 10-15 e 15-20cm), do solo de várzea... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Percolação de herbicidas; Residual; Preparo de solo; HPLC; Oryza sativa. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000600005 |
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| Matallo,M.B; Almeida,S.D.B; Cerdeira,A.L; Franco,D.A; Blanco,F.M.G; Menezes,P.T.C; Luchini,L.C; Moura,M.A.M; Duke,S.O. |
| A better method for determination of shikimate in plant tissues is needed to monitor exposure of plants to the herbicide glyphosate [N-(phosphonomethyl)glycine] and to screen the plant kingdom for high levels of this valuable phytochemical precursor to the pharmaceutical oseltamivir. A simple, rapid, and efficient method using microwave-assisted extraction (MWAE) with water as the extraction solvent was developed for the determination of shikimic acid in plant tissues. High performance liquid chromatography was used for the separation of shikimic acid, and chromatographic data were acquired using photodiode array detection. This MWAE technique was successful in recovering shikimic acid from a series of fortified plant tissues at more than 90% efficiency... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glyphosate; Green chemistry; Herbicide; HPLC. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582009000500012 |
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| Registros recuperados: 89 | |
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